1、 SCI experiment outsourcing

How long does it take to create SCI research articles in the life science/medicine field?

The influence factor is about 20 points or more, such as CNS and its sub journals.

The main body of the document has many experimental methods and data, while the supplementary materials have more data. There are 6 to 7 numbers in the text, and each number has an average of 6 to 7 experiments. The supplement is calculated according to 6 figures, and each figure has an average of 4 experiments. Therefore, at least 60 experiments are required for top-level papers.

Secondly, a typical experiment requires at least three independent iterations.

Moreover, many experiments know that once completed, they will not succeed. They all sublimate from failure, failure and failure. Upgrade from junior high school and enter the department to fight monsters (plasma, cells, mice). The success rate of senior brothers and sisters gradually increased, with an average level of 1/3. Then, from the beginning of the experiment to the main experimental results, 60 × three × 3=540 experiments, how long will it take? Hopefully this will take several years.

Second grade thick paper

The influencing factors are 9 to 15 points, such as JCI, intestinal tract, cancer research, etc.

There are 6 to 7 numbers in the text, and each number has an average of 5 experiments. The supplementary materials shall be calculated by 5 digits. On average, there are 3 experiments per graph. In this case, thick paper needs to be tested at least 45 times (3 times). In the above independent iteration, the success rate of the experiment is calculated to be 1/3, so the experiment must be executed from the beginning to the main experimental results × three × 3=405 experiments. If all goes well, it will take two years.

Third grade, powerful thesis

Papers with impact factors of 5 to 7, such as Development and Cancer Bulletin.

There are 5 to 6 numbers in the text, and each number has an average of 4 experiments. The supplementary materials are calculated as 4 numbers, and each number is tested twice on average. Secondly, a strong paper requires at least 28 experiments, which is 3. In the above independent iteration, the success rate of the experiment is 1/3, and 28 × three × 3=252 experiments.

Fourth grade, popular papers

The impact factor is less than 5 points, such as Plos One, BBRC, etc.

There are 4 to 5 figures in the text, each figure has 3 experiments on average, and the supplementary materials are calculated as 3 figures. On average, there are two experiments in each graph. In this case, a popular paper requires at least 18 experiments and at least 3 experiments. For multiple independent iterations, the success rate of the experiment is calculated as 1/3, and 18 × three × 3=162 experiments. If the experiment is successful, it will take about 10 months to one year.

However, before starting the topic, you must read a lot of literature, and literature reading in junior high school is like a book, which is slow and time-consuming.

Research dogs often encounter unexpected results and need to fine tune or significantly adjust their research direction. It is unlikely to be the same as the assumptions made in the topic, and the experiment will take longer.

Look at the top papers and heavyweight papers. Many of them use multiple transgenic mice, which is time-consuming.

It usually takes more than half a year for top papers and heavyweight papers to be written, submitted, reviewed, modified from small to large, and approved, let alone rejected and submitted.

Brother takes out a private dry substance:

1. Work hard and run the laboratory frequently. After enrollment, graduate students will further go to the laboratory, and upgrade to fight against monsters from the morning (extract cultured cells, plasmids, conduct electrophoresis, and conduct Western analysis) and practice basic skills. However, it is recommended that you understand the general research direction of teachers as soon as possible and choose the small direction you are interested in.

2. The experimental platform is very important

What is a good experimental platform?

An excellent animal laboratory has a brilliant tutor, a good scientific research environment and many basic experimental tools.

Advanced experimental methods and experience are inherited in the same way. Of course, a complete set of equipment and instruments is also essential.

A good laboratory will save you a lot of innovation time. When your experiment is muddy, a competent lecturer will catch up with you in time and lead to failure as soon as possible. You can find it.

For example, if you want to view NF Ƙ B activity, you have a Ƙ For the plasmid related to B, your predecessors and sisters have completed EMSA, so the experiment should be completed in a few minutes. If not, you will need to write an email to request plasmids or build your own. This will take some time. Is it really painful that you chose the right platform?

3. At the beginning of the experiment, please do not put the eggs in the basket, open the double bases (study in two small directions), keep your hands stiff and adjust the focus during the study.

4. What if young researchers (with abundant funds but lots of education and medical work) have no time or students? Biological companies are emerging. They become small bosses, spend money on virus packaging, hire people to carry out basic experiments, outsource experiments, etc. (but look for things that can be trusted. At present, the audit work is strict!).